Lab 6 isolation of chromosomal dna

Pipette it into a microcentrifuge tube and dispose of the micropipette tip. Dispose of the tip after each use. Dispose of the tip.

Lab 6 isolation of chromosomal dna

Certain sources of DNA should not be used, such as: Your family pet, Fido the dog Your little sister's big toe Bugs you caught in the yard Step 1: The blender separates the pea cells from each other, so you now have a really thin pea-cell soup. Soapy Peas Pour your thin pea-cell soup through a strainer into another container like a measuring cup.

Add 2 tablespoons liquid detergent about 30ml and swirl to mix. Let the mixture sit for minutes.


Why am I adding detergent? Enzyme Power Add a pinch of enzymes to each test tube and stir gently. If you stir too hard, you'll break up the DNA, making it harder to see.

Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution. Pour until you have about the same amount of alcohol in the tube as pea mixture.

Alcohol is less dense than water, so it floats on top. Look for clumps of white stringy stuff where the water and alcohol layers meet. What is that Stringy Stuff? DNA is a long, stringy molecule. The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules!

DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved. This is called precipitation. The physical force of the DNA clumping together as it precipitates pulls more strands along with it as it rises into the alcohol.

You can use a wooden stick or a straw to collect the DNA.

Lab 6 isolation of chromosomal dna

If you want to save your DNA, you can transfer it to a small container filled with alcohol. Now that you've successfully extracted DNA from one source, you're ready to experiment further. Try these ideas or some of your own: Experiment with other DNA sources.Bacterial Genomic DNA Isolation Teacher s Guidebook (Cat.

# BE æ) This includes wearing lab coats, gloves and safety goggles. The salt solution aids in the precipitation of the DNA. 6. Centrifuge the tube for 5 minutes at 5,xg to pellet the cell debris. Transfer the. 43 Experiment 1 Isolation of Escherichia coli Chromosomal DNA In this lab we will isolate E.

coli chromosomal DNA for use in several experiments throughout the semester. The basic protocol involves lysing the cells with lysozyme and SDS. Peptidoglycan strips away the. Proteins are contaminating agents in any type of DNA isolation so as in plasmid DNA isolation also.

They can interfere with the final product and result with low yield. SDS is used to denature the proteins and facilitate the DNA purification process. timberdesignmag.coml Considerations for Plasmid DNA Purification 6 A.

Bacterial Growth and Culture Conditions 6 H. Fixed-Tissue Genomic DNA Isolation 23 timberdesignmag.comc DNA Purification Protocols Featuring the separation of plasmid DNA from the chromosomal DNA and cellular RNA of .

A series of lab exercises giving instructions for the extraction of DNA from several different starting materials. The exercise is designed for the grade level. The .

Lab 6 isolation of chromosomal dna

Experiment 2 Plasmid DNA Isolation, Restriction Digestion and Gel Electrophoresis 6 mixing the enzyme and DNA with a buffer specific for the enzyme of choice. Once all the will result in shearing of the genomic DNA, leaving free chromosomal fragments to contaminate the plasmid DNA.

Chromosomal DNA isolation from E. coli - OpenWetWare